Title | μMap-Red: Proximity Labeling by Red Light Photocatalysis. |
Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Buksh BF, Knutson SD, Oakley JV, Bissonnette NB, Oblinsky DG, Schwoerer MP, Seath CP, Geri JB, Rodriguez-Rivera FP, Parker DL, Scholes GD, Ploss A, MacMillan DWC |
Journal | J Am Chem Soc |
Volume | 144 |
Issue | 14 |
Pagination | 6154-6162 |
Date Published | 2022 04 13 |
ISSN | 1520-5126 |
Keywords | Animals, Biotin, Light, Membrane Proteins, Mice, Proteomics, Staining and Labeling |
Abstract | Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited SnIV chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling in vitro through several layers of tissue, and we then apply our platform in cellulo to label EGFR microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models. |
DOI | 10.1021/jacs.2c01384 |
Alternate Journal | J Am Chem Soc |
PubMed ID | 35363468 |
Grant List | R35 GM134897 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States P30 CA072720 / CA / NCI NIH HHS / United States |